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MEK-2 (phospho Thr394) rabbit pAb
SKU: orb764231
Featured
Description
Images & Validation
−Item 1 of 4
| Tested Applications | ELISA, IHC-P, IP, WB |
|---|---|
| Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunoprecipitation: 2-5 ug/mg lysate. ELISA: 1/10000. Not yet tested in other applications. |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human MEK2 around the phosphorylation site of Thr394. AA range:261-310 |
| Molecular Weight | 44kD |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
| Concentration | 1 mg/ml |
| Disclaimer | For research use only |
Alternative Names
−Anti-MAP2K2 antibody, anti-MEK2 antibody, anti-MKK2 antibody, anti-PRKMK2 antibody, anti-Dual specificity mitogen-activated protein kinase kinase 2 antibody, anti-MAP kinase kinase 2 antibody, anti-MAPKK 2 antibody, anti-ERK activator kinase 2 antibody, anti-MAPK/ERK kinase 2 antibody, anti-MEK 2 antibody
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Western Blot analysis of various cells using Phospho-MEK-2 (T394) Polyclonal Antibody diluted at 1:2000.
Western Blot analysis of VEC cells using Phospho-MEK-2 (T394) Polyclonal Antibody diluted at 1:2000.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using MEK2 (Phospho-Thr394) Antibody. The picture on the right is blocked with the phospho peptide.
Western blot analysis of lysates from ovary cancer, using MEK2 (Phospho-Thr394) Antibody. The lane on the right is blocked with the phospho peptide.
Quick Database Links
Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
IP
Immunoprecipitation
MEK-2 (phospho Thr394) rabbit pAb (orb764231)
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