Rabbit anti-CD70 Recombinant Monoclonal Antibody

SKU: orb1784577

Description

Images & Validation

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Item 1 of 3

Tested Applications	ICC, IHC, WB
Dilution range	WB: 1:1000, IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections, ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections
Reactivity	Human
Application Notes	Format: Whole IgG

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Recombinant
Clone No.	BLR266L
Immunogen	Residues 50-193 (ECD)
Target	CD70

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Whole IgG
Buffer/Preservatives	Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide
Concentration	500 µg/ml
Disclaimer	For research use only

Alternative Names

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surface antigen CD70; tumor necrosis factor ligand superfamily member 7; tumor necrosis factor ligand 8A; TNFSF7; Ki-24 antigen; CD27LG; CD27-L; CD27L; CD27 ligand; CD antigen CD70; TNLG8A; CD70 antigen

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Rabbit anti-CD70 Recombinant Monoclonal Antibody

Detection of human CD70 by western blot. Samples: Whole cell lysate (50 µg) from Jurkat, 786-O (10 µg) , HEK293T, THP-1, and HeLa cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD70 recombinant monoclonal antibody (orb1784577) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

Detection of human CD70 by immunohistochemistry. Sample: FFPE section of human lymph node with metastatic lung cancer. Antibody: Rabbit anti-CD70 recombinant monoclonal antibody (orb1784577). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of human CD70 by immunocytochemistry. Sample: FFPE section of 786-O cells. Antibody: Rabbit anti-CD70 recombinant monoclonal antibody (orb1784577). Secondary: HRP-conjugated goat anti-rabbit IgG.

Quick Database Links

Gene Symbol

CD70

UniProt

P32970

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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ICC

Immunocytochemistry

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