Rabbit anti-Phospho MCM2 (S53) Recombinant Monoclonal Antibody

SKU: orb1519724

Description

MCM2, Phospho (S53) Antibody

Images & Validation

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Item 1 of 3

Tested Applications	ICC, IHC, WB
Dilution range	WB - 1:1,000; IHC - 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.; ICC - 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
Reactivity	Human, Mouse
Application Notes	Format: Whole IgG

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Recombinant
Clone No.	BLR108H
Immunogen	Surrounding Serine 53
Target	MCM2, Phospho (S53)

Storage & Handling

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Storage	2 - 8°C
Form/Appearance	Whole IgG
Buffer/Preservatives	Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide
Concentration	250 µg/ml
Disclaimer	For research use only

Alternative Names

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D3S3194; MITOTIN; minichromosome maintenance protein 2 homolog; minichromosome maintenance deficient 2 (mitotin); nuclear protein BM28; DFNA70; cell devision cycle-like 1; CDCL1; cdc19; CCNL1; BM28; DNA replication licensing factor MCM2; cyclin-like 1

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Rabbit anti-Phospho MCM2 (S53) Recombinant Monoclonal Antibody

Detection of human Phospho-MCM2 (S53) by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-MCM2 antibody used for IP. Immunoprecipitates where either treated with phosphatase (+) or mock treated (-). For blotting immunoprecipitated Phospho-MCM2 (S53) , orb1519724 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Total MCM2.

Detection of mouse Phospho-MCM2 (S53) by western blot. Samples: Whole cell lysate (10 µg) from BW5147.3, TCMK-1, mIMCD-3, A20, NIH 3T3, and CT26 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-Phospho-MCM2 (S53) recombinant monoclonal antibody (orb1519724) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-Actin recombinant monoclonal.

Detection of human Phospho-MCM2 (S53) by western blot. Samples: Whole cell lysate (10 µg) from HDLM-2, HeLa, Jurkat, MCF-7, MOLT-4, Malme-3M, HEK293T, and Raji cells prepared using NETN lysis buffer. Antibody: Rabbit anti-Phospho-MCM2 (S53) recombinant monoclonal antibody (orb1519724) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal.

Quick Database Links

Gene Symbol

MCM2, Phospho (S53)

UniProt

P49736

RefSeq (Protein):NP_004517.2

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_004517.2

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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ICC

Immunocytochemistry

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