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Rabbit anti-PRP6 Antibody
SKU: orb1526015
Description
Images & Validation
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| Tested Applications | IHC, IP, WB |
|---|---|
| Dilution range | WB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate; IHC - 1:200 - 1:1,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. |
| Reactivity | Human, Mouse |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Immunogen | Between 150 and 200 |
| Target | PRP6 |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | 2 - 8°C |
|---|---|
| Form/Appearance | Whole IgG |
| Buffer/Preservatives | Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide |
| Concentration | 200 µg/ml |
| Disclaimer | For research use only |
Alternative Names
−PRP6 pre-mRNA processing factor 6 homolog; U5-102K; U5-102 kDa protein; U5 snRNP-associated 102 kDa protein; TOM; ANT1; SNRNP102; RP60; putative mitochondrial outer membrane protein import receptor; Prp6; pre-mRNA-processing factor 6; p102 U5 small nuclear ribonucleoprotein particle-binding protein; hPrp6; ANT-1; C20orf14; Androgen receptor N-terminal domain-transactivating protein 1; androgen receptor N-terminal domain transactivating protein-1; PRP6 homolog
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Detection of human and mouse PRP6 by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) , HEK293T (T; 50 µg) , and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-PRP6 antibody orb1526015 used for WB at 0.04 µg/ml (A) and 0.4 µg/ml (B) and used for IP at 3 µg/mg lysate. PRP6 was also immunoprecipitated by rabbit anti-PRP6 antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).
Detection of human PRP6 by immunohistochemistry.Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-PRP6 (Cat. No. orb1526015) used at a dilution of 1: 1, 000 (0.2 µg/ml). Detection: DAB
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_036601.2 |
|---|
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IP
Immunoprecipitation
Rabbit anti-PRP6 Antibody (orb1526015)
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