Raf-B (phospho Ser446) rabbit pAb

SKU: orb770155

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Description

Rabbit polyclonal antibody to Raf-B (phospho-Ser446)

Images & Validation

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Item 1 of 4

Tested Applications	ELISA, IHC-P, WB
Dilution range	Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.
Reactivity	Human, Mouse

Key Properties

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Clonality	Polyclonal
Isotype	IgG
Immunogen	The antiserum was produced against synthesized peptide derived from human B-RAF around the phosphorylation site of Ser446. AA range:411-460
Molecular Weight	110
Purification	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
Concentration	1 mg/ml
Disclaimer	For research use only

Alternative Names

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Anti-BRAF antibody, anti-BRAF1 antibody, anti-RAFB1 antibody, anti-Serine/threonine-protein kinase B-raf antibody, anti-Proto-oncogene B-Raf antibody, anti-p94 antibody, anti-v-Raf murine sarcoma viral oncogene homolog B1 antibody

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Western Blot analysis of 293 cells using Phospho-Raf-B (S446) Polyclonal Antibody diluted at 1:1000.

Western blot analysis of K562 293T lysis using Phospho-Raf-B (S446) antibody. Antibody was diluted at 1:1000.

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using B-RAF (Phospho-Ser446) Antibody.

Immunohistochemistry analysis of paraffin-embedded human brain, using B-RAF (Phospho-Ser446) Antibody. The picture on the right is blocked with the phospho peptide.

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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