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Recombinant Human CALCRL&RAMP1 Heterodimer Protein (C-Fc)
SKU: orb2992774
Description
Images & Validation
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Key Properties
−| Expression System | Human Cells |
|---|---|
| Biological Origin | Human |
| Biological Activity | ELISA: Immobilized Recombinant Human CALCRL&RAMP1 Heterodimer Protein (C-Fc) (Cat#orb2992774) at 2μg/ml (100 μl/well) can bind Anti-Human CALCRL&RAMP1 mAb (Erenumab)*. *:Biotinylated by NHS-biotin prior to testing. The ED50 of Anti-Human CALCRL&RAMP1 mAb(Erenumab) is 16.13 ng/ml. (Regularly tested). BLI: Loaded Anti-Human CALCRL&RAMP1 mAb (Human IgG2) on SA Biosensor, can bind Recombinant Human CALCRL&RAMP1 Heterodimer Protein (C-Fc) (Cat#orb2992774) with an affinity constant of 0.987 nM as determined in BLI assay. (Regularly tested) |
| Tag | C-Fc&C-Fc |
| Molecular Weight | Predicted: 41.1&37.2 KDa. Observed: (36-43)&(46-66) KDa, reducing conditions |
| Expression Region | Glu23-Ile146&Cys27-Ser117 |
| Purity | SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. (QC verified) |
| Endotoxins | < 1 EU/µg as determined by LAL test. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
|---|---|
| Form/Appearance | Lyophilized from a 0.2 μm filtered solution of 50mM HEPES, 150mM NaCl, pH 7.4. |
| Reconstitution | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Disclaimer | For research use only |
Alternative Names
−CALCRL&RAMP1 complex Protein

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Recombinant Human CALCRL&RAMP1 Heterodimer Protein (C-Fc) (orb2992774)
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