14-3-3 beta antibody [N1C2]

SKU: orb556840

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Description

Rabbit polyclonal antibody to 14-3-3 beta

Research Area

Epigenetics, Infectious Diseases

Images & Validation

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Item 1 of 4

Tested Applications	ICC, IHC-P, WB
Dilution Range	WB: 1:5000-1:20000 , IF: 1:100-1:1000 , IHC-P: 1:100-1:1000
Reactivity	Human, Mouse, Rat
Application Notes	Positive control: Mouse brain , 293T , A431 , HeLa , HepG2

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Recombinant protein encompassing a sequence within the center region of human 14-3-3 beta. The exact sequence is proprietary.
Target	14-3-3 beta
Molecular Weight	28
Purity	Purified by antigen-affinity chromatography.
Purification	Purified by antigen-affinity chromatography.
Conjugation	Unconjugated

Storage & Handling

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Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°lC or below. Avoid multiple freeze-thaw cycles.
Form/Appearance	Liquid: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Buffer/Preservatives	0.01% Thimerosal. 0.1M Tris-Glycine (pH7), 10% Glycerol
Concentration	1 mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein beta , GW128 , HEL-S-1 , HS1 , KCIP-1 , YWHAA

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Immunofluorescence analysis of HeLa cells using 14-3-3 beta antibody

Immunohistochemical staining of rat cervix using 14-3-3 beta antibody

Immunohistochemical staining of SW480 Xenograft using 14-3-3 beta antibody

Western blot analysis of mouse brain using 14-3-3 beta antibody

Quick Database Links

Gene Symbol

14-3-3 beta

UniProt

P31946

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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ICC

Immunocytochemistry

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