Acetylated Lysine Antibody

SKU: orb182143

Description

Rabbit Anti-Acetylated Lysine Polyclonal

Research Area

Protein Biochemistry, Signal Transduction

Images & Validation

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Item 1 of 4

Tested Applications	ELISA, ICC, IF, IP, WB
Dilution Range	WB (1:250), ICC/IF (1:100)
Reactivity	All
Application Notes	A 1/250 dilution was sufficient to detect the acetylated histone from TSA treated mouse spleen cell in western blot analysis.

Key Properties

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Host	Rabbit
Clonality	Polyclonal
Immunogen	Acetylated KLH Conjugated
Target	Acetylated Lysine
Purification	Protein A Purified
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	PBS, 50% glycerol, 0.09% sodium azide Storage buffer changes when conjugated
Concentration	1 mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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N6-Acetyl-L-lysine, N-epsilon-Acetyl-L-lysine, Nepsilon-Acetyllysine, N6-Acetyllysine, Acetyllysine

Quality Guarantee

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Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Acetylated Lysine Polyclonal Antibody. Tissue: Heat Shocked Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Acetylated Lysine Polyclonal Antibody at 1:100 for 12 hours at 4°C. Secondary Antibody: R-PE Goat Anti-Rabbit (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Nucleus. Cytoplasm. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-Acetylated Lysine Antibody. (C) Composite. Heat Shocked at 42°C for 1h.

Western blot analysis of Bovine serum albumin showing detection of Acetylated Lysine protein using Rabbit Anti-Acetylated Lysine Polyclonal Antibody. Primary Antibody: Rabbit Anti-Acetylated Lysine Polyclonal Antibody at 1:1000. Acetylated lysine in BSA (Left) and Acetylated BSA (Right).

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Acetylated Lysine Polyclonal Antibody. Tissue: Heat Shocked Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Acetylated Lysine Polyclonal Antibody at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Nucleus. Cytoplasm. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-Acetylated Lysine Antibody. (C) Composite. Heat Shocked at 42°C for 1h.

Western blot analysis of Mouse Spleen lysates showing detection of Acetylated Lysine protein using Rabbit Anti-Acetylated Lysine Polyclonal Antibody. Primary Antibody: Rabbit Anti-Acetylated Lysine Polyclonal Antibody at 1:1000.

Quick Database Links

Gene Symbol

Acetylated Lysine

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Protocol Information

Western Blot (IB, immunoblot)

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Immunofluorescence

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ICC

Immunocytochemistry

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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Immunoprecipitation

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