AFAP Rabbit Polyclonal Antibody

SKU: orb2045

Description

Research Area

Actin Crosslinking, Cell Biology, Cytoplasmic

Images & Validation

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Item 1 of 1

Tested Applications	WB
Dilution range	WB=1:500-2000
Reactivity	Human
Predicted Reactivity	Bovine, Canine, Equine, Mouse, Rat, Sheep

Related Conjugates & Formulations

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Biotin

HRP

IRDye800

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from human AFAP (277-345/730aa)
Target	AFAP1
Molecular Weight	81 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

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110 kDa actin filament associated protein; 110 kDa actin filament-associated protein; Actin filament associated protein 1; Actin filament associated protein 110 kDa; Actin filament-associated protein 1; AFAP 1; AFAP 110; AFAP; AFAP-110; Afap1; AFAP1_HUMAN.

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Sample: Lane 1: Human HeLa cell lysates, Lane 2: Human DU145 cell lysates, Lane 3: Human MCF-7 cell lysates, Primary: Anti-AFAP (orb2045) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 81 kDa, Observed band size: 120 kDa.

Quick Database Links

Gene Symbol

AFAP1

UniProt

Q8N556

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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