Aloxistatin

SKU: orb1301127

Description

Aloxistatin (E64d) is a cell-permeable, irreversible inhibitor of cysteine proteases, including calpain, and inhibits platelet aggregation. It is widely used in research to study protease function in cellular models and in vivo, particularly in neuroscience and cardiovascular studies.

Research Area

Infectious Disease & Virology, Protein Biochemistry

Images & Validation

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Key Properties

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CAS Number	88321-09-9
MW	342.43
Purity	99.69% (May vary between batches)
Formula	C₁₇H₃₀N₂O₅
SMILES	CCOC(=O)[C@H]1O[C@@H]1C(=O)N[C@@H](CC(C)C)C(=O)NCCC(C)C
Target	Cysteine Protease,SARS-CoV
Solubility	DMSO:10.73 mg/mL (31.33 mM)

Bioactivity

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In Vivo	Aloxistatin (100 mg/kg, p.o.) strongly inhibits the cathepsin B&L activities in the skeletal muscle, heart and liver of hamsters. In spinal cord injury (SCI) rats, Aloxistatin provides neuroprotection in SCI lesion and penumbra. Aloxistatin reduces brain amyloid-β and improves memory deficits in Alzheimer's disease animal models by inhibiting cathepsin B activity. Read more...
In Vitro	Aloxistatin can enter the intact platelet and inhibit proteolysis by inhibiting calpain. Aloxistatin blunts Parathyroid hormone (PTH)-induced cell proliferation and inhibits differentiation osteoblasts in vitro. Read more...
Cell Research	Aloxistatin (E64d) is dissolved in DMSO and stored, and then diluted with appropriate medium (final DMSO 0.1 %) before use. Cell proliferation and apoptosis are assessed by staining for a proliferation marker, Ki67, or an apoptotic marker, cleaved caspase 3, following the protocol described above for the polarity markers. MCF10 variants are grown in 3D rBM overlay cultures for 4 days and are treated with 0.1 % DMSO, 5 μM CA074Me or 5 μM Aloxistatin. The percentage of structures that are positive for Ki67 or cleaved caspase 3 is determined by counting a total of 100 structures on two separate coverslips with a Zeiss Axiophot epifluorescent microscope. Structures are considered Ki67 positive if they contained at least one cell staining for Ki67. Structures are considered to be caspase 3 positive if they contained at least one cell that is positive for cleaved caspase 3 and the positive cell(s) is not localized in the center of a developing lumen. Read more...

Storage & Handling

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Storage	Powder: -20°C for 3 years \| In solvent: -80°C for 1 year \| Shipping with blue ice/Shipping at ambient temperature.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Loxistatin, Inhibitor, inhibit, Aloxistatin, Cathepsin, E64c ethyl ester, E64d, CysteineProtease, Cysteine Protease, Cysteine protease, SARS coronavirus, SARSCoV, SARS-CoV

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Compound Identifiers

PubChem CID

65663 ↗

Key Properties

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No computed properties available.

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Aloxistatin (orb1301127)

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Purity:	>98% (HPLC)
CAS Number:	88321-09-9
MW:	342.4
Formula:	C₁₇H₃₀N₂O₅

Aloxistatin

Description

Research Area

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Key Properties

Bioactivity

Storage & Handling

Alternative Names

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