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AMY2B Antibody
SKU: orb1270657
Featured
Description
Research Area
Cancer Biology, Metabolism Research, Signal Transduction
Images & Validation
−Item 1 of 4
| Tested Applications | FC, IF, IHC-P, WB |
|---|---|
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit Ig |
| Immunogen | This AMY2B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 74-100 amino acids from the N-terminal region of human AMY2B. |
| Target | AMY2B |
| Molecular Weight | 58 kDa |
| Purification | This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Supplied in PBS with 0.09% (W/V) sodium azide. |
| Concentration | batch dependent |
| Disclaimer | For research use only |
Alternative Names
−Alpha-amylase 2B,4-alpha-D-glucan glucanohydrolase 2B, Carcinoid alpha-amylase, AMY2B
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Western blot analysis in HL-60, CEM and K562 cell line lysates (35 ug/lane).
AMY2B antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Confocal immunofluorescent analysis of AMY2B Antibody with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
FC
Flow Cytometry
IF
Immunofluorescence
AMY2B Antibody (orb1270657)
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