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Anti-CD80 [7C10]
SKU: orb1089959
Description
Research Area
Epigenetics, Infectious Diseases
Images & Validation
−Item 1 of 2
| Tested Applications | Blocking, ELISA |
|---|---|
| Reactivity | Human |
Key Properties
−| Host | Monkey |
|---|---|
| Clonality | Monoclonal |
| Isotype | Monkey IgG |
| Clone No. | 7C10 |
| Immunogen | This antibody was generated by immunization of cynomolgus monkeys with human B7.1 antigen. |
| Target | CD80 |
| Purity | Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | PBS with 0.02% Proclin 300. |
| Concentration | 1 mg/ml |
| Disclaimer | For research use only |
Alternative Names
−B7.1; CD28L; CD28LG; B7-1; B7; BB1; B7/BB1;T-lymphocyte activation antigen CD80; Activation B7-1 antigen; CTLA-4 counter-receptor B7.1; CTLA4L; CTLA-4L
Quality Guarantee
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![Anti-CD80 [7C10]](/images/pub/media/catalog/product/NewWebsite/35/orb1089959_1.png)
Flow cytometry using the Anti-CD80 antibody 7C10. Paraformaldehyde fixed Daudi cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (orb256458; isotype control, black line) or the rabbit IgG version of 7C10 (orb1089959, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.
![Anti-CD80 [7C10]](/images/pub/media/catalog/product/NewWebsite/35/orb1089959_2.png)
Immunofluorescence staining of Daudi cells with anti-CD80 7C10. Immunofluorescence analysis of paraformaldehyde fixed Daudi cells on Shi-fix™ cover-slips stained with the chimeric rabbit IgG version of 7C10 (orb1089959) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb1089959, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (orb256458) followed by staining with Alexa Fluor® 488 secondary antibody.
Quick Database Links
Gene Symbol
CD80
UniProt
UniProt Details
− No UniProt data available
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Datasheet
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Anti-CD80 [7C10] (orb1089959)
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