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FOXH1 Antibody
SKU: orb2649555
Description
Images & Validation
−Item 1 of 2
| Tested Applications | IF, WB |
|---|---|
| Dilution range | WB: WB (1/500 - 1/1000), IF/IC (1/50 - 1/200), IF: WB (1/500 - 1/1000), IF/IC (1/50 - 1/200) |
| Reactivity | Human |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Clone No. | FOXH1 |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Disclaimer | For research use only |
Alternative Names
−FAST1; FAST2; Forkhead box protein H1; Forkhead activin signal transducer 1; Fast-1; hFAST-1; Forkhead activin signal transducer 2; Fast-2
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Western blot analysis of FOXH1 expression in SGC7901 (A), Myla2059 (B) whole cell lysates.
Immunofluorescent analysis of FOXH1 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4°C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IF
Immunofluorescence
FOXH1 Antibody (orb2649555)
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