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HLA Class I Antibody
SKU: orb44076
Description
Images & Validation
−Item 1 of 3
| Tested Applications | FC, IP, WB |
|---|---|
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Clonality | Monoclonal |
| Isotype | Mouse IgG1 |
| Clone No. | MEM-147 |
| Immunogen | PHA-activated peripheral blood lymphocytes |
| Target | HLA Class I |
| Purification | Purified by protein-A affinity chromatography. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Concentration | 1 mg/ml |
| Disclaimer | For research use only |
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Western blotting analysis of human Ramos B cell line lysate (1% laurylmaltoside); non-reduced sample, immunostained by mAb MEM-147 and goat anti-mouse IgG (H+L)-HRP conjugate.
Separation of human leukocytes stained using anti-HLA Class I (MEM-147) purified antibody (concentration in sample 1.67 µg/ml, GAM PE, red-filled) from human leukocytes unstained by primary antibody (GAM PE, black-dashed) in flow cytometry analysis (surface staining).
Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-HLA Class I (MEM-147) purified antibody (concentration in sample 1.67 µg/ml) GAM PE.
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Protocol Information
WB
Western Blot (IB, immunoblot)
FC
Flow Cytometry
IP
Immunoprecipitation
HLA Class I Antibody (orb44076)
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