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HLA-E Antibody
SKU: orb44422
Description
Research Area
Epigenetics, Immunology, Infectious Diseases
Images & Validation
−Item 1 of 3
| Tested Applications | FC, IHC-P, IP |
|---|---|
| Reactivity | Human, Primate |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Clonality | Monoclonal |
| Isotype | Mouse IgG1 |
| Clone No. | MEM-E/06 |
| Immunogen | Bacterially expressed recombinant HLA-E refolded with beta2-microglobulin and peptide. |
| Target | HLA-E |
| Purification | Purified by protein-A affinity chromatography. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Concentration | 1 mg/ml |
| Disclaimer | For research use only |
Alternative Names
−HLA class I histocompatibility antigen, alpha chain E, MHC class I antigen E
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Separation of human monocytes (red-filled) from HLA-E negative blood debris (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-HLA-E (MEM-E/06) purified antibody (concentration in sample 0.56 µg/ml, GAM APC).
Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-HLA-E (MEM-E/06) purified antibody (concentration in sample 0.56 µg/ml, GAM APC).
Immunohistochemistry staining of human spleen (paraffin sections) with anti-HLA-E (clone MEM-E/06).
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Protocol Information
IHC-P
Immunohistochemistry Paraffin
FC
Flow Cytometry
IP
Immunoprecipitation
HLA-E Antibody (orb44422)
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