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ATF-2 (phospho Ser62) rabbit pAb Antibody
SKU: orb764141
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Description
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−Item 1 of 4
| Tested Applications | ELISA, IF, IHC, IP, WB |
|---|---|
| Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunoprecipitation: 2-5 ug/mg lysate. ELISA: 1/20000. Not yet tested in other applications. |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human ATF2 around the phosphorylation site of Ser62 or 44. AA range:29-78 |
| Molecular Weight | 52kD |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
| Concentration | 1 mg/ml |
| Disclaimer | For research use only |
Alternative Names
−Anti-ATF2 antibody, anti-CREB2 antibody, anti-CREBP1 antibody, anti-Cyclic AMP-dependent transcription factor ATF-2 antibody, anti-cAMP-dependent transcription factor ATF-2 antibody, anti-Activating transcription factor 2 antibody, anti-Cyclic AMP-responsive element-binding protein 2 antibody, anti-CRE antibody
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Western Blot analysis of various cells using Phospho-ATF-2 (S62) Polyclonal Antibody diluted at 1:1000.
Western Blot analysis of HeLa cells using Phospho-ATF-2 (S62) Polyclonal Antibody diluted at 1:1000.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using ATF2 (Phospho-Ser62 or 44) Antibody.
Western blot analysis of lysates from HeLa cells treated with TNF-alpha, using ATF2 (Phospho-Ser62 or 44) Antibody. The lane on the left is blocked with the phospho peptide.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
IP
Immunoprecipitation
ATF-2 (phospho Ser62) rabbit pAb Antibody (orb764141)
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