ATP7A, Mouse & Rat, pAb Antibody

SKU: orb1108738

Description

Rabbit polyclonal antibody to ATP7A

Images & Validation

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Tested Applications	IF, IHC-Fr, PS, WB
Dilution range	For immunohistochemistry, and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, in vitro dilutions have to be optimized in user’s experimental setting.
Reactivity	Mouse, Rat
Application Notes	F: Postfixed overnight in paraformaldehyde (4°C), followed by incubation in 25% sucrose in PBS + 0.02% NaN3 (4°C). Sections (12 µm) were cut on a cryostat and collected on gelatin-coated slides P: Fixed in Bouin's solution for 20h (4°C) and paraffin embedded. Endogenous peroxidase activity was blocked with 0.1% H2O2 in methanol (15 min). W: A reduced sample treatment and SDS-Page was used. Read more...

Key Properties

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Clonality	Polyclonal
Isotype	Rabbit Ig
Immunogen	Peptide (Asp1475-Leu1492) situated at the C-terminus of mouse (NM009726) and rat (NM052803) ATP7A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	1 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.
Buffer/Preservatives	0.2 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.
Disclaimer	For research use only

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-Fr

Immunohistochemistry Frozen

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Immunofluorescence

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