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Bax Antibody

SKU: orb2645115

Description

Recognizes a protein of 21kDa, identified as the Bax protein. This mAb is highly specific to Bax and shows no cross-reaction with Bcl-2 or Bcl-X protein. Bcl-2 blocks cell death following a variety of stimuli. Bax has extensive amino acid homology with Bcl-2 and it homodimerizes and forms heterodimers with Bcl-2. Overexpression of Bax accelerates apoptotic death induced by cytokine deprivation in an IL-3 dependent cell line, and Bax also counters the death repressor activity of Bcl-2.

Images & Validation

Tested ApplicationsFACS, IF, IHC-P, WB
Dilution rangeWestern blot: 1-2ug/ml,Flow cytometry: 1-2ug/10^6 cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
ReactivityHuman
Application Notes
The optimal dilution of the anti-Bax antibody for each application should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

Antibody TypePrimary Antibody
HostMouse
ClonalityMonoclonal
IsotypeMouse IgG1, kappa
Clone No.SPM336
ImmunogenAmino acids 3-16 (GSGEQPRGGGPTSS) from the human protein were used as the immunogen for this anti-Bax antibody.
PurificationProtein G affinity chromatography
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
DisclaimerFor research use only

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Quality Guarantee

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Bax Antibody

Immunofluorescent staining of fixed human Jurkat cells with anti-Bax antibody (clone SPM336, green) and NucSpot nuclear stain (red).

Bax Antibody

Western blot testing of HeLa cell lysate with anti-Bax antibody (clone SPM336). Observed molecular weight ~21 kDa.

Bax Antibody

IHC analysis of formalin-fixed, paraffin-embedded human Hodgkin's lymphoma stained with anti-Bax antibody (clone SPM336).

Bax Antibody

Flow cytometry testing of fixed human Jurkat cells with anti-Bax antibody (clone SPM336); Red=isotype control, Blue= anti-Bax antibody.

UniProt Details

No UniProt data available

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC-P
Immunohistochemistry Paraffin
View Protocol
FACS
Fluorescence-Activated Cell Sorting (FC, Flow cytometry)
View Protocol
IF
Immunofluorescence
View Protocol

Bax Antibody (orb2645115)

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Available Sizes

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100 μg
¥ 9,750.00
DispatchUsually dispatched within 5-10 working days
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