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Bax Antibody
Description
Images & Validation
−| Tested Applications | FACS, IF, IHC-P, WB |
|---|---|
| Dilution range | Western blot: 1-2ug/ml,Flow cytometry: 1-2ug/10^6 cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | Mouse IgG1, kappa |
| Clone No. | SPM336 |
| Immunogen | Amino acids 3-16 (GSGEQPRGGGPTSS) from the human protein were used as the immunogen for this anti-Bax antibody. |
| Purification | Protein G affinity chromatography |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Disclaimer | For research use only |
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Immunofluorescent staining of fixed human Jurkat cells with anti-Bax antibody (clone SPM336, green) and NucSpot nuclear stain (red).

Western blot testing of HeLa cell lysate with anti-Bax antibody (clone SPM336). Observed molecular weight ~21 kDa.

IHC analysis of formalin-fixed, paraffin-embedded human Hodgkin's lymphoma stained with anti-Bax antibody (clone SPM336).

Flow cytometry testing of fixed human Jurkat cells with anti-Bax antibody (clone SPM336); Red=isotype control, Blue= anti-Bax antibody.
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Bax Antibody (orb2645115)
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