Recombinant Bcl-2 Antibody / Rabbit Monoclonal

SKU: orb2640843

Description

This antibody recognizes a protein of 25-26kDa, identified as the Bcl-2 alpha oncoprotein. It shows no cross-reaction with Bcl-x or Bax protein. Expression of bcl-2 alpha oncoprotein inhibits the programmed cell death (apoptosis). In most follicular lymphomas, neoplastic germinal centers express high levels of bcl-2 alpha protein, whereas the normal or hyperplastic germinal centers are negative. Consequently, this antibody is valuable when distinguishing between reactive and neoplastic follicular proliferation in lymph node biopsies. It may also be used in distinguishing between those follicular lymphomas that express bcl-2 protein and the small number in which the neoplastic cells are bcl-2 negative.

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Tested Applications	IHC-P, WB
Dilution range	Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT
Reactivity	Human
Application Notes	The optimal dilution of the recombinant Bcl-2 antibody for each application should be determined by the researcher.

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Recombinant
Isotype	Rabbit IgG, kappa
Clone No.	ARBC2-2R
Immunogen	Recombinant full length human protein was used as the immunogen for this recombinant Bcl-2 antibody.
Purification	Protein A affinity chromatography
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Disclaimer	For research use only

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Recombinant Bcl-2 Antibody / Rabbit Monoclonal

IHC testing of FFPE human spleen tissue with recombinant Bcl-2 antibody (clone ARBC2-2R). HIER: boil tissue sections in 1mM EDTA buffer, pH7.5-8.5, for 10-20 min followed by cooling at RT for 20 min.

Western blot testing of human MCF7 cell lysate with recombinant Bcl2 antibody (clone ARBC2-2R). Expected molecular weight ~26 kDa.

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UniProt

P10415

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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