Bcl6 Antibody

SKU: orb749773

Description

Recognizes a protein of 95kDa, which is identified as Bcl-6. Antibody to bcl-6 is helpful in a number of diagnostic settings: (1) In the differential diagnosis of small B-cell lymphoma. Follicular lymphoma will show bcl-6 (and CD10) positivity whereas other small B-cell lymphomas are usually negative. (2) Bcl-6 is an important prognostic marker in diffuse large B-cell lymphomas (DLBCL), where CD10, bcl-6 and MUM1/IRF4 are used to identify germinal center and activated B-cell phenotypes. (3) Bcl-6 can be valuable in distinguishing classical Hodgkin lymphoma from nodular lymphocyte predominant Hodgkin lymphoma (NLPHL). The Reed-Sternberg cells of classical Hodgkin lymphoma are bcl-6 negative whereas the large (L&H) cells of NLPHL are bcl-6 positive. In contrast, anti-Bcl-6 rarely stains mantle-cell lymphoma and MALT lymphoma.

Research Area

Cancer, Epigenetics, Immunology

Images & Validation

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Tested Applications	IHC-P
Dilution range	Immunohistochemistry (FFPE): 0.125-0.25ug/ml for 30 min at RT
Reactivity	Human, Mouse
Application Notes	Optimal dilution of the anti-Bcl6 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9, for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. Read more...

Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Clonality	Monoclonal
Isotype	Mouse IgG1, kappa
Clone No.	SPM602
Immunogen	Recombinant human protein was used as the immunogen for the anti-Bcl6 antibody.
Purification	Protein G affinity chromatography
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Disclaimer	For research use only

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IHC testing of formalin-fixed, paraffin-embedded human lymphoma stained with anti-Bcl6 antibody (clone SPM602).

IHC testing of formalin-fixed, paraffin-embedded human tonsil stained with anti-Bcl6 antibody (clone SPM602).

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UniProt

P41182

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Protocol Information

IHC-P

Immunohistochemistry Paraffin

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Bcl6 Antibody (orb749773)

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Applications:	FC, IF
Reactivity:	Human
Species/Host:	Mouse
Clonality:	Monoclonal
Conjugation:	Unconjugated

Applications:	ELISA, IF, IHC, WB
Reactivity:	Human, Mouse, Rat
Clonality:	Polyclonal
Conjugation:	Unconjugated

Applications:	ELISA, IF, IHC, WB
Reactivity:	Human, Mouse, Rat
Clonality:	Polyclonal
Conjugation:	Unconjugated

Applications:	FC, IF
Reactivity:	Human
Species/Host:	Mouse
Clonality:	Monoclonal
Conjugation:	Unconjugated

Applications:	ELISA, IF, IHC-Fr, IHC-P, WB
Predicted Reactivity:	Bovine, Equine, Porcine, Rabbit, Rat
Reactivity:	Human, Mouse
Species/Host:	Rabbit
Clonality:	Polyclonal
Conjugation:	Unconjugated

Bcl6 Antibody

Description

Research Area

Images & Validation

Key Properties

Storage & Handling

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Bcl6 Antibody (orb749773)

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