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Human CALCRL&RAMP1
SKU: orb2992606
Description
Images & Validation
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Key Properties
−| Expression System | Human Cells |
|---|---|
| Biological Origin | Human |
| Biological Activity | ELISA: Immobilized Human CGRP Antibody (Erenumab, Research Grade) (Cat#orb2992589) at 2 μg/ml (100 μl/well) can bind Biotinylated Human CALCRL&RAMP1 Heterodimer Protein (C-Fc-Avi)(Cat#orb2992606). (Regularly tested). BLI: Loaded Biotinylated Human CALCRL&RAMP1 Heterodimer Protein (Cat#orb2992606) on SA Biosensor, can bind Human CGRP Antibody (Erenumab, Research Grade) (Cat#orb2992589) with an affinity constant of 2.08 pM as determined in BLI assay. (Regularly tested) |
| Tag | C-Fc-Avi |
| Molecular Weight | Predicted: 42.9&39 KDa. Observed: (40-44)&(50-65) KDa, reducing conditions |
| Expression Region | Glu23-Ile146&Cys27-Ser117 |
| Purity | SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. (QC verified) |
| Endotoxins | ＜ 1 EU/μg as determined by LAL test. |
Storage & Handling
−| Storage | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
|---|---|
| Form/Appearance | Lyophilized from a 0.2 μm filtered solution of 50 mM HEPES, 150 mM NaCl, pH 7.4 |
| Reconstitution | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Disclaimer | For research use only |
Alternative Names
−CALCRL&RAMP1 Heterodimer Protein
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Human CALCRL&RAMP1 (orb2992606)
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