c-Kit (phospho Tyr936) rabbit pAb

SKU: orb768896

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Description

Rabbit polyclonal antibody to c-Kit (phospho-Tyr936)

Images & Validation

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Item 1 of 3

Tested Applications	ELISA, IHC-P, WB
Dilution range	Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.
Reactivity	Human, Mouse

Key Properties

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Clonality	Polyclonal
Isotype	IgG
Immunogen	The antiserum was produced against synthesized peptide derived from human KIT around the phosphorylation site of Tyr936. AA range:906-955
Molecular Weight	145
Purification	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
Concentration	1 mg/ml
Disclaimer	For research use only

Alternative Names

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Anti-KIT antibody, anti-SCFR antibody, anti-Mast/stem cell growth factor receptor Kit antibody, anti-SCFR antibody, anti-Piebald trait protein antibody, anti-PBT antibody, anti-Proto-oncogene c-Kit antibody, anti-Tyrosine-protein kinase Kit antibody, anti-p145 c-kit antibody, anti-v-kit Hardy-Zuckerman 4 feline sarcoma viral onco antibody

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Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Immunohistochemistry analysis of paraffin-embedded human heart, using KIT (Phospho-Tyr936) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from HepG2 cells treated with EGF 200ng/ml 30', using KIT (Phospho-Tyr936) Antibody. The lane on the right is blocked with the phospho peptide.

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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