CAPRIN2 Rabbit Polyclonal Antibody

SKU: orb156264

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Description

Research Area

Cell Biology, Cell Differentiation, Cytoplasmic

Images & Validation

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Item 1 of 4

Tested Applications	IF, IHC-Fr, IHC-P, WB
Dilution range	WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Reactivity	Human, Rat
Predicted Reactivity	Equine, Gallus, Mouse, Porcine, Sheep

Related Conjugates & Formulations

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Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from human CAPRIN2 (101-200/1127aa)
Target	CAPRIN2
Molecular Weight	126 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

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C1q domain-containing protein 1; C1QDC1; CAPR2_HUMAN; Caprin family member 2; Caprin-2; caprin2; Cytoplasmic activation/proliferation-associated protein 2; EEG 1; EEG1; Gastric cancer multidrug resistance-associated protein; KIAA1873; Protein EEG-1; RNA granule protein 140; RNG140.

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Paraformaldehyde-fixed, paraffin embedded (rat brain tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (CAPRIN2) Polyclonal Antibody, Unconjugated (orb156264) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat cerebellum), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (CAPRIN2) Polyclonal Antibody, Unconjugated (orb156264) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: U251 (Human) Cell Lysate at 30 ug, Lane 2: U87MG (Human) Cell Lysate at 30 ug, Lane 3: Huvec (Human) Cell Lysate at 30 ug, Primary: Anti-CAPRIN2 (orb156264) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 120-140 kD, Observed band size: 140 kD.

Sample: U87MG (Human) Cell Lysate at 30 ug, U2OS (Human) Cell Lysate at 30 ug, Primary: Anti-CAPRIN2 (orb156264) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 126 kD, Observed band size: 126 kD.

Quick Database Links

Gene Symbol

CAPRIN2

UniProt

Q6IMN6

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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IHC-Fr

Immunohistochemistry Frozen

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Immunofluorescence

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