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CD14 Antibody (Macrophage Marker)

SKU: orb2641057

Description

Recognizes a protein of 55kDa, identified as Cluster of Differentiation 14 (also known lipopolysaccharide receptor or LPSR). CD14 is expressed strongly on monocytes and macrophage and weakly on the surface of neutrophils. It is anchored to cells by linkage to glycosylphosphatidylinositol (GPI) and functions as a high affinity receptor for complexes of LPS and LPS binding protein (LBP). Soluble CD14, also binding to LPS, acts at physiological concentration as an LPS agonist and has, at higher concentrations, an LPS antagonizing effect in cell activation.

Images & Validation

Tested ApplicationsIHC-P, WB
Dilution rangeWestern blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
ReactivityHuman
Application Notes
Variations in protocols, secondaries and substrates may require the CD14 antibody to be titered up or down for optimal performance.

Key Properties

Antibody TypePrimary Antibody
HostMouse
ClonalityMonoclonal
IsotypeMouse IgG1, kappa
Clone No.LPSR/2408
ImmunogenA human recombinant partial protein (within amino acids 25-148) was used as immunogen for this CD14 antibody.
PurificationProtein G affinity chromatography
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
DisclaimerFor research use only

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CD14 Antibody (Macrophage Marker)

IHC testing of FFPE human tonsil with CD14 antibody (clone LPSR/2408). HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH9, for 10-20 min followed by cooling at RT for 20 min.

CD14 Antibody (Macrophage Marker)

IHC testing of FFPE human lymph node with CD14 antibody (clone LPSR/2408). HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH9, for 10-20 min followed by cooling at RT for 20 min.

CD14 Antibody (Macrophage Marker)

Analysis of HuProt (TM) microarray containing more than 19, 000 full-length human proteins using CD14 antibody (clone LPSR/2408). These results demonstrate the foremost specificity of the LPSR/2408 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

CD14 Antibody (Macrophage Marker)

SDS-PAGE analysis of purified, BSA-free CD14 antibody (clone LPSR/2408) as confirmation of integrity and purity.

CD14 Antibody (Macrophage Marker)

Western blot testing of human A549 cell lysate with CD14 antibody (clone LPSR/2408). Expected molecular weight 40-55 kDa depending on glycosylation level.

UniProt Details

No UniProt data available

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC-P
Immunohistochemistry Paraffin
View Protocol

CD14 Antibody (Macrophage Marker) (orb2641057)

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Available Sizes

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100 μg
¥ 9,750.00
DispatchUsually dispatched within 5-10 working days
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