CD98 Antibody / SLC3A2

SKU: orb2637933

Description

CD98 exits as a heterodimer containing a disulphide-linked glycosylated heavy chain and a non-glycosylated light chain. It is a member of the solute carrier family and encodes a cell surface, transmembrane protein. The protein exists as the heavy chain of a heterodimer, covalently bound through disulfide bonds to one of several possible light chains. The encoded transporter plays a role in regulation of intracellular calcium levels and transports L-type amino acids. Alternatively spliced transcript variants, encoding different isoforms, have been characterized.

Images & Validation

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Tested Applications	FACS, Functional Studies, IF
Dilution range	Comitogenic with soluble anti-CD2 and immobilized anti-CD3 mAbs,Flow cytometry: 0.5-1ug/10^6 cells,Immunofluorescence: 0.5-1ug/ml
Reactivity	Human
Application Notes	The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CD98 antibody to be titered up or down for optimal performance.

Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Clonality	Monoclonal
Isotype	Mouse IgG1, kappa
Clone No.	UM7F8
Immunogen	The Molt-13 T cell line was used as the immunogen for this CD98 antibody.
Purification	Protein G purified CD98 antibody
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	1X PBS, pH 7.4
Disclaimer	For research use only

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SDS-PAGE Analysis of Purified, BSA-Free CD98 Antibody (clone UM7F8). Confirmation of Integrity and Purity of the Antibody.

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Protocol Information

FACS

Fluorescence-Activated Cell Sorting (FC, Flow cytometry)

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Immunofluorescence

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