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KRT14 Antibody (Biotin)
SKU: orb751277
Description
Research Area
Cell Biology, Stem Cell & Developmental Biology
Images & Validation
−Item 1 of 3
| Tested Applications | FC, IF, IHC |
|---|---|
| Dilution range | Flow Cytometry (2-4ug/million cells); Immunofluorescence (2-4ug/ml); ,Immunohistochemistry (Formalin-fixed) (2-4ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined. |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Host | Mouse |
|---|---|
| Clonality | Monoclonal |
| Isotype | IgG3 |
| Clone No. | LL002 |
| Immunogen | A synthetic peptide of 15 amino acid from the C-terminus of human keratin 14. |
| Target | KRT14 |
| Source/Expression System | Bioreactor |
| Molecular Weight | ~50kDa |
| Purification | Protein G |
| Conjugation | Biotin |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml. |
| Buffer/Preservatives | Antibody purified from Bioreactor Concentrate by Protein A/G and conjugated to Biotin. Prepared in 10mM PBS with 0.05% rAlbumin and 0.05% azide. |
| Concentration | Purified Ab conjugated to Biotin |
| Disclaimer | For research use only |
Alternative Names
−Anti-CK-14 antibody, anti-Dowling Meara antibody, anti-ebs3 antibody, anti-ebs4 antibody, anti-Epidermolysis Bullosa Simplex antibody, anti-Keratin Type I Cytoskeletal 14 antibody, anti-Koebner antibody, anti-NFJ antibody
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Flow Cytometric Analysis of trypsinized MeOH-fixed HeLa cells using Cytokeratin 14 Mouse Monoclonal Antibody (LL002) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).
Immunofluorescence Analysis of A549 cells labeling CK14 with Cytokeratin 14 Mouse Monoclonal Antibody (LL002) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Reddot (Red).
SDS-PAGE Analysis Purified Cytokeratin 14 Mouse Monoclonal Antibody (LL002). Confirmation of Purity and Integrity of Antibody.
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KRT14 Antibody (Biotin) (orb751277)
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