Cytokeratin 8 (phospho Ser73) rabbit pAb Antibody

SKU: orb764171

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Description

Rabbit polyclonal antibody to Cytokeratin 8 (phospho-Ser73)

Images & Validation

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Item 1 of 4

Tested Applications	ELISA, IF, IHC, WB
Dilution range	Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.
Reactivity	Human, Mouse

Key Properties

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Clonality	Polyclonal
Isotype	IgG
Immunogen	The antiserum was produced against synthesized peptide derived from human Keratin 8 around the phosphorylation site of Ser73. AA range:41-90
Molecular Weight	55
Purification	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
Concentration	1 mg/ml
Disclaimer	For research use only

Alternative Names

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Anti-KRT8 antibody, anti-CYK8 antibody, anti-Keratin antibody, anti- type II cytoskeletal 8 antibody, anti-Cytokeratin-8 antibody, anti-CK-8 antibody, anti-Keratin-8 antibody, anti-K8 antibody, anti-Type-II keratin Kb8 antibody

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Cytokeratin 8 (phospho Ser73) rabbit pAb Antibody

Western Blot analysis of various cells using Phospho-Cytokeratin 8 (S73) Polyclonal Antibody diluted at 1:500.

Western Blot analysis of 293 cells using Phospho-Cytokeratin 8 (S73) Polyclonal Antibody diluted at 1:500.

Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Keratin 8 (Phospho-Ser73) Antibody.

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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Immunofluorescence

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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