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E2F1 Rabbit Polyclonal Antibody
SKU: orb573731
Description
Research Area
Cancer Biology, Cell Biology, Epigenetics & Chromatin, Immunology & Inflammation, Molecular Biology, Signal Transduction
Images & Validation
−Item 1 of 3
| Tested Applications | WB |
|---|---|
| Reactivity | Human |
| Predicted Reactivity | Human |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Target | E2F1 |
| Molecular Weight | 47kDa |
| Purification | Affinity Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| Concentration | 0.5 mg/ml |
| Disclaimer | For research use only |
Alternative Names
−RBP3, E2F-1, RBAP1, RBBP3
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Lanes: 1. 30 ug Cytosol extract: Untransfected cells 2. 30 ug Cytosol extract: Cells transfected with control (non-targeting) siRNA 3. 30 ug Cytosol extract: Cells transfected with anti-E2F1-siRNA 4. 30 ug Cytosol extract: Cells transfected with anti-E2F1-siRNA, Primary Antibody dilution: 1:2000, Secondary Antibody: Goat anti-rabbit HRP, Secondary Antibody dilution: 1:2000, Gene Name: E2F1.
Rabbit Anti-E2F1 Antibody, Catalog Number: orb573731, Formalin Fixed Paraffin Embedded Tissue: Human Lymph Node Tissue, Observed Staining: Cytoplasm, Primary Antibody Concentration: 1:100, Other Working Concentrations: 1:600, Secondary Antibody: Donkey anti-Rabbit-Cy3, Secondary Antibody Concentration: 1:200, Magnification: 20X, Exposure Time: 0.5-2.0 sec.
WB Suggested Anti-E2F1 Antibody, Titration: 1.0 ug/ml, Positive Control: Fetal Lung.
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E2F1 Rabbit Polyclonal Antibody (orb573731)
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