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FM1-43

SKU: orb2308707

Description

FM1-43 is a lipophilic styryl dye used to label and study hair cell mechanotransduction channels, neuropeptide vesicles, and cytotoxicity. dFdCTP trisodium, the active triphosphate metabolite of Gemcitabine, acts as a chain terminator to inhibit DNA synthesis. These small molecules are applied in auditory research, oncology studies, and various in vitro and in vivo experimental models.
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Images & Validation

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FM1-43

Key Properties

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CAS Number149838-22-2
MW611.54
Purity99.78%
FormulaC30H49Br2N3
SMILES[Br-].[Br-].CCCCN(CCCC)c1ccc(C=Cc2cc[n+](CCC[N+](CC)(CC)CC)cc2)cc1
TargetOthers
SolubilityDMSO:40 mg/mL (65.41 mM)

Bioactivity

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Cell Research
Cell staining experiment a. Solution preparation: 1. Mother solution preparation: Prepare 5 mM mother solution with DMSO, store at -20℃ or -80℃ in the dark after aliquoting. 2. Working solution preparation: Use preheated HBSS solution to prepare 5-20 μM FM 1-43 working solution (select the appropriate working solution concentration according to experimental requirements, and prepare it for immediate use). b. Cell staining 1. Suspended cell staining 1.1 Collect cells by centrifugation and wash twice with PBS for 5 minutes each time. Add 1 mL FM1-43 working solution and incubate at room temperature for 5-30 minutes. 1.2 Centrifuge at 400 g for 3-4 minutes and discard the supernatant. 1.3 Add PBS to wash cells twice for 5 minutes each time. 1.4 Resuspend cells with 1 mL serum-free medium or PBS and observe using a fluorescence microscope or flow cytometer. 2. Adherent cell staining 2.1 Culture iron-adherent cells on sterile slides, discard the culture medium, and remove excess residual liquid. 2.2 Add 100 μL of dye working solution, gently shake to completely cover the cells, and incubate for 5-30 minutes. 2.3 Aspirate the dye working solution, wash with culture medium 2-3 times, 5 minutes each time, and observe using a fluorescence microscope or flow cytometer.
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Storage & Handling

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Storagestore at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

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FM1 43, FM143

Similar Products

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  • FM1-43

    FM1-43 [orb2900271]

    >98% (HPLC)

    149838-22-2

    1 g, 500 mg, 2 mg, 5 mg, 10 mg, 25 mg, 50 mg, 100 mg
Quality Guarantee

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Compound Identifiers

PubChem CID
2733618 ↗

Key Properties

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No computed properties available.

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FM1-43 (orb2308707)

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% DMSO +
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% Tween 80 +
%

Available Sizes

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1 mg
¥ 2,080.00
5 mg
¥ 4,290.00
10 mg
¥ 6,240.00
25 mg
¥ 9,490.00
50 mg
¥ 13,260.00
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