Frizzled-7 rabbit pAb Antibody

SKU: orb765248

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Description

Rabbit polyclonal antibody to Frizzled-7

Images & Validation

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Item 1 of 4

Tested Applications	ELISA, FC, IF, WB
Dilution Range	WB 1:500-2000;Flow Cyt 1:50-200;IF/ICC 1:100-500;ELISA 1:5000-20000
Reactivity	Human, Mouse

Key Properties

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Clonality	Polyclonal
Isotype	IgG
Immunogen	The antiserum was produced against synthesized peptide derived from human FZD7. AA range:45-94
Molecular Weight	64
Purification	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
Concentration	1 mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Anti-FZD7 antibody, anti-Frizzled-7 antibody, anti-Fz-7 antibody, anti-hFz7 antibody, anti-FzE3 antibody

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Western Blot analysis of various cells using Frizzled-7 Polyclonal Antibody diluted at 1:2000.

Immunofluorescence analysis of A549. 1, primary Antibody (red) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit IgG (H&L) - Alexa Fluor 594 Secondary antibody was diluted at 1:1000 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min.

Western Blot analysis of HELA cells using Frizzled-7 Polyclonal Antibody diluted at 1:2000.

Immunofluorescence analysis of MCF7 cells, using FZD7 Antibody. The picture on the right is blocked with the synthesized peptide.

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Protocol Information

Western Blot (IB, immunoblot)

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Flow Cytometry

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Immunofluorescence

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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