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GIT1 rabbit pAb
SKU: orb765301
Featured
Description
Images & Validation
−Item 1 of 4
| Tested Applications | ELISA, IHC-P, WB |
|---|---|
| Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications. |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human GIT1. AA range:561-610 |
| Molecular Weight | 95 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
| Concentration | 1 mg/ml |
| Disclaimer | For research use only |
Alternative Names
−Anti-GIT1 antibody, anti-ARF GTPase-activating protein GIT1 antibody, anti-ARF GAP GIT1 antibody, anti-Cool-associated and tyrosine-phosphorylated protein 1 antibody, anti-CAT-1 antibody, anti-CAT1 antibody, anti-G protein-coupled receptor kinase-interactor 1 antibody, anti-GRK-interacting protein 1 antibody
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Western Blot analysis of various cells using GIT1 Polyclonal Antibody.
Western Blot analysis of HepG2 cells using GIT1 Polyclonal Antibody.
Western blot analysis of the lysates from 293 cells using GIT1 antibody.
Immunohistochemical analysis of paraffin-embedded human spleen tissue. 1, primary Antibody was diluted at 1:200 (4°C overnight). 2, Sodium citrate pH 6.0 was used for antigen retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
GIT1 rabbit pAb (orb765301)
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