Goat anti-PAD4 / PADI4 Antibody

SKU: orb18935

Description

Goat polyclonal antibody to PADI4

Images & Validation

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Item 1 of 3

Tested Applications	ELISA, IF, IHC
Dilution range	ELISA: 1:64000, WB: 0.3-1 μg/ml, IHC-P: 5 μg/ml
Reactivity	Human
Application Notes	ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Approx 74kDa band observed in Human Spleen and Liver lysates (calculated MW of 74.1kDa according to NP_036519.2). In transfected HEK293 transiently expressing PADI4 a band of approx. 75kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.3-1 μg/ml..

Key Properties

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Host	Goat
Clonality	Polyclonal
Target	PAD4 / PADI4
Molecular Weight	74.1
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Disclaimer	For research use only

Alternative Names

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anti PADI4 antibody, anti PAD antibody, anti PDI4 antibody, anti PDI5 antibody, anti PADI5 antibody, anti peptidyl arginine deiminase, type IV antibody, anti protein-arginine deiminase antibody, anti peptidyl arginine deiminase, type V antibody

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HEK293 overexpressing PADI4 (RC206501) and probed with orb18935 (mock transfection in first lane), tested by Origene.

Western blot analysis of Human Spleen lysate using PADI4 antibody

Immunohistochemical staining of Human Spleen using PADI4 antibody

Quick Database Links

Gene Symbol

PAD4 / PADI4

NCBI Gene ID

23569

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

IHC

Immunohistochemistry

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Immunofluorescence

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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