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PU.1 Antibody
SKU: orb19964
Description
Research Area
Cancer
Images & Validation
−Item 1 of 3
| Tested Applications | ELISA, IHC, WB |
|---|---|
| Dilution range | ELISA: 1:32000, WB: 0.03-0.1 μg/ml, IHC-P: 3.75 μg/ml |
| Reactivity | Human |
| Predicted Reactivity | Rat |
| Application Notes |
Key Properties
−| Host | Goat |
|---|---|
| Clonality | Polyclonal |
| Target | PU.1 |
| Molecular Weight | 31.2; 31.1 |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Disclaimer | For research use only |
Alternative Names
−anti spleen focus forming virus (SFFV) proviral integration oncogene spi1 antibody, anti hCG_25181 antibody, anti SFPI1 antibody, anti SPI-1 antibody, anti SPI-A antibody, anti 31 kDa transforming protein antibody
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orb19964 (0.03ug/ml) staining of DAUDI lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemical staining of Human Colon using SPI1 antibody
Immunohistochemical staining of Human Spleen using SPI1 antibody
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
PU.1 Antibody (orb19964)
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