HMGB1, Human, mAb 5H6 Antibody

SKU: orb1108820

Description

Mouse monoclonal antibody to HMGB1

Images & Validation

−

Tested Applications	FC, IF, WB
Dilution Range	For flow cytometry, immunofluorescence and Western blot, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
Reactivity	Human
Application Notes	FC: For staining cells were permeabilized with buffer containing 0.5% saponin. The cells were fixed in 0.01% formaldehyde before staining. Hamster IgG isotype control is used as a negative control. IF: NIH-3T3 cells were fixed with 2% PFA (15 min). After fixation, cells were washed with PBS and incubated for 5 min at 4°C with permeabilization buffer . The cells were blocked with 1% BSA/10% goat serum in PBS for 10 min and were incubated with HMGB1 mAb 5H6 (10 µg/ml) overnight at 4°C. The secondary antibody was biotinylated goat anti-hamster IgG at a 1:500 dilution for 1 hour. Finally, streptavidin was used at a 1:500 dilution for 30 min. W: A band is expected at ~30 kDa using reducing conditions. For non-reducing conditions a band is expected at both 27 kDa and 30 kDa. Read more...

Key Properties

−

Clonality	Monoclonal
Isotype	Armenian hamster IgG
Conjugation	Unconjugated

Storage & Handling

−

Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	1 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.
Buffer/Preservatives	0.2 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

−

Sulfoglucuronyl Carbohydrate Binding Protein, Amphoterin, SBP-1

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Quick Database Links

UniProt

P09429

UniProt Details

−

No UniProt data available

Documents Download

Datasheet

Product Information

Download

Request a Document

Protocol Information

Western Blot (IB, immunoblot)

Flow Cytometry

Immunofluorescence