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HSP110 Antibody
SKU: orb1822412
Description
Research Area
Cancer Biology, Cell Biology, Protein Biochemistry, Signal Transduction
Images & Validation
−Item 1 of 2
| Tested Applications | ICC, IF, IHC, WB |
|---|---|
| Dilution range | WB (1:1000), IHC (1:100); optimal dilutions for assays should be determined by the user. |
| Reactivity | Bovine, Fish, Hamster, Human, Monkey, Mouse, Rat, Sheep, Yeast |
| Application Notes |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Immunogen | Synthetic peptide derived from the sequence of hamster HSP110; sequence identical to human and mouse |
| Target | HSP110 |
| Molecular Weight | 110kDa |
| Purification | Protein A Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | PBS pH 7.2, 50% glycerol, 0.09% sodium azide *Storage buffer changes when conjugated |
| Concentration | 1 mg/ml |
| Disclaimer | For research use only |
Alternative Names
−HSPH1, HSP110, HSP105, Heat shock protein 105 kDa, Heat shock 110 kDa protein, KIAA0201, Heat shock 105kDa alpha, heat shock 105kDa beta, HSP105A, HSP105B, NY CO 25
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Immunohistochemistry analysis using Rabbit Anti-HSP110 Polyclonal Antibody. Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative Solution. Primary Antibody: Rabbit Anti-HSP110 Polyclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at RT. Localization: Positive epidermal, dermal and muscle staining.
Western blot analysis of Human Cell line lysates showing detection of HSP110 protein using Rabbit Anti-HSP110 Polyclonal Antibody. Load: 15 μgprotein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-HSP110 Polyclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Gene Details
− No NCBI Gene data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_006635.3 |
|---|
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
ICC
Immunocytochemistry
HSP110 Antibody (orb1822412)
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