IP-10, Human, mAb 6D4 Antibody

SKU: orb1109035

Description

Mouse monoclonal antibody to IP-10

Images & Validation

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Tested Applications	FC, IA, IF, IHC-Fr, WB
Dilution range	Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
Reactivity	Human
Application Notes	FC: Antibody 6D4 stains human CXCL10. For intracellular staining activated plasmacytoid dendritic cells were fixed with 2% paraformaldehyde and permeabilized with 0.5% saponin. All steps were performed in presence of 0.5 M EDTA to avoid cell aggregation (Ref.3) W: Tissue was homogenized in loading sample buffer and subjected to SDS-PAGE. The band size is ~9 kDa (Ref.4). IHC-F: Tissue sections were fixed 10 minutes in acetone and pretreated with avidin/biotin blocking kit. As positive control chronically inflamed liver was used and as negative control normal liver and isotype matched antibody (Ref.4). Read more...

Key Properties

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Clonality	Monoclonal
Isotype	Mouse IgG2a
Immunogen	Recombinant human IP-10
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	1 ml (100 µg/ml) 0.2 um filtered protein G purified antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.
Buffer/Preservatives	Quantities >500 µg will be send without preservative and carrier free. Quantities
Disclaimer	For research use only

Alternative Names

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10 kDa interferon gamma-induced protein, CXCL10, Small-inducible cytokine B10 Gene name: CXCL10

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-Fr

Immunohistochemistry Frozen

Flow Cytometry

Immunofluorescence