MEK2 N-Term Antibody

SKU: orb420227

Description

MEK2 antibody

Images & Validation

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Item 1 of 2

Tested Applications	ELISA, WB
Dilution range	ELISA: 1:40,000, WB: 1µg/mL
Reactivity	Human, Mouse, Rat
Application Notes	Anti-MEK 2 (MOUSE) antibody has been tested in ELISA, PAGE-MAP, and Western Blotting. Specific conditions of reactivity should be optimized by the end user. Expect a band of approximately 44 kDa.

Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Clonality	Monoclonal
Isotype	IgG
Clone No.	19G10.F1.E2
Immunogen	Anti-MEK2 Monoclonal Antibody was produced in mice by repeated immunizations with synthetic peptide corresponding to amino acid residues near the N-terminus conjugated to KLH.
Purity	This protein A purified mouse monoclonal antibody reacts specifically with human MEK2. Anti-MEK2 is purified from tissue culture supernatant by protein A purification. Cross reactivity is expected to occur with human, mouse, and rat based on sequence identity of the peptide immunogen. This antibody does not react with the MEK1 isoform.
Conjugation	Unconjugated

Storage & Handling

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Storage	Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Form/Appearance	Liquid (sterile filtered)
Buffer/Preservatives	0.01% (w/v) Sodium Azide
Concentration	1.0 mg/mL
Dry Ice Shipping	Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
Disclaimer	For research use only

Alternative Names

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mouse anti-MEK2 Antibody, MAP2K2, MEK, MEK 2, MKK2, PRKMK2 ,CFC4, MEK-2 Antibody

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PAGE-MAP (microsphere affinity proteomics) of Mouse Anti-MEK2/MAP2K2 Antibody (orb420226). Antibody array western blot binding of gelfree size separated fractions of multiple lysates (solid lines) and shotgun mass spectroscopy identification (dashed lines) of the target band run in parallel correlate confirming the specificity of this antibody against MEK2/MAP2K2.

Western Blot of Anti-MEK2 Antibody. Lane 1: MEK-1 recombinant protein. Lane 2: MEK-2 recombinant protein. Load: 50 ng per lane. Primary Antibody: Anti-MEK2 supernatant clone neat over night at 4°C. Secondary Antibody: Anti-mouse HRP at 1:40000 dilution.

Quick Database Links

UniProt

P36507

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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