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MEK2 (MAP2K2) Antibody (N-term)
SKU: orb1928730
Description
Images & Validation
−Item 1 of 3
| Tested Applications | IF, WB |
|---|---|
| Dilution range | WB - 1:1000, IF - 1:10-50 |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Molecular Weight | 44424 Da |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Disclaimer | For research use only |
Alternative Names
−MEK2, MKK2, PRKMK2
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Quality Guarantee
Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].
MEK2 (MAP2K2) Antibody (N-term) western blot analysis in Hela, MCF-7, rat C6 cell line and mouse Liver, rat Brain tissue lysates (35 ug/lane).This demonstrates the MEK2 (MAP2K2) antibody detected the MEK2 (MAP2K2) protein (arrow).
All lanes: Anti-MEK2 (MAP2K2) Antibody (N-term) at 1:1000 dilution. Lane 1: Hela whole cell lysate. Lane 2: MOLT-4 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 44 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Fluorescent confocal image of U251 cell stained with MEK2 (MAP2K2) Antibody (N-term).U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with MEK2 (MAP2K2) primary antibody (1: 25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min).MEK2 (MAP2K2) immunoreactivity is localized to Cytoplasm significantly.
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WB
Western Blot (IB, immunoblot)
IF
Immunofluorescence
MEK2 (MAP2K2) Antibody (N-term) (orb1928730)
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