MerTK/Tyro3 (phospho Tyr749/681) rabbit pAb

SKU: orb767352

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Description

Rabbit polyclonal antibody to MerTK/Tyro3 (phospho-Tyr749/681)

Images & Validation

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Tested Applications	ELISA, IHC-P, WB
Dilution range	Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.
Reactivity	Human, Mouse, Rat

Key Properties

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Clonality	Polyclonal
Isotype	IgG
Immunogen	The antiserum was produced against synthesized peptide derived from human MER/SKY around the phosphorylation site of Tyr749/681. AA range:716-765
Molecular Weight	97
Purification	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
Concentration	1 mg/ml
Disclaimer	For research use only

Alternative Names

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Anti-MERTK antibody, anti-MER antibody, anti-Tyrosine-protein kinase Mer antibody, anti-Proto-oncogene c-Mer antibody, anti-Receptor tyrosine kinase MerTK antibody, anti-TYRO3 antibody, anti-BYK antibody, anti-DTK antibody, anti-RSE antibody, anti-SKY antibody, anti-Tyrosine-protein kinase receptor TYRO3 antibody, anti-Tyrosine-protein kinase DTK antibody, anti-Tyrosine- antibody

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MerTK/Tyro3 (phospho Tyr749/681) rabbit pAb

Western Blot analysis of 3T3 cells using Phospho-MerTK/Tyro3 (Y749/681) Polyclonal Antibody diluted at 1:500.

Western Blot analysis of 293T/mouse-heart using Antibody diluted at 1:1000. Secondary antibody was diluted at 1:20000.

Immunohistochemistry analysis of paraffin-embedded human brain, using MER/SKY (Phospho-Tyr749/681) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from NIH/3T3 cells treated with EGF 200ng/ml 5', using MER/SKY (Phospho-Tyr749/681) Antibody. The lane on the right is blocked with the phospho peptide.

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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