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NANOG Antibody
SKU: orb1939430
Description
Research Area
Cancer Biology, Signal Transduction, Stem Cell & Developmental Biology
Images & Validation
−Item 1 of 3
| Tested Applications | IHC-P, WB |
|---|---|
| Dilution Range | WB - 1:500-1000, IHC-P - 1:100-500 |
| Reactivity | Human, Mouse |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1,k |
| Molecular Weight | 34620 Da |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
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NANOG western blot analysis in K562 cell line lysates (35 μg/lane). This demonstrates the NANOG antibody detected the NANOG protein (arrow).
NANOG Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of NANOG Antibody for immunohistochemistry. Clinical relevance has not been evaluated.
Western blot analysis of anti-NANOG monoclonal antibody by NANOG recombinant protein. NANOG (NANOG + His tag) (arrow) was detected using the Mab.
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UniProt Details
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
NANOG Antibody (orb1939430)
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