NCAM/CD56 Antibody

SKU: orb1537023

Description

NCAM/CD56 Antibody

Images & Validation

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Item 1 of 2

Tested Applications	ELISA, IHC-P, WB
Dilution Range	IHC-P (5 µg/ml), Peptide-ELISA (1:32000), WB (0.01 - 0.03 µg/ml)
Reactivity	Bovine, Human, Rat
Application Notes	Further information: Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx 170kD band observed in Human Brain (Cerebellum, Cerebral and Frontal Cortex) lysates (calculated MW of 94.6kD according to NP_851996.2). The observed molecular weight corresponds to the glycosylated form. Recommended concentration: 0.01-0.03 ug/ml.

Key Properties

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Host	Goat
Clonality	Polyclonal
Immunogen	Peptide with sequence C-KASWTRPEKQETLD, from the internal region of the protein sequence according to NP_851996.2.
Target	NCAM / CD56
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	TBS, pH 7.3, 0.02% Sodium Azide, 0.5% rAlbumin
Concentration	0.5 mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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NCAM1, CD56, CD56 antigen, MSK39, NCAM-1, N-CAM-1, NCAM

Quality Guarantee

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Human Adrenal: Formalin-Fixed, Paraffin-Embedded (FFPE)

NCAM1 antibody (0.01 ug/ml) staining of Human Frontal Cortex lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Quick Database Links

Gene Symbol

NCAM / CD56

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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