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Ngp Antibody
SKU: orb420373
Description
Images & Validation
−Item 1 of 3
| Tested Applications | IF, WB |
|---|---|
| Dilution range | IF: 1:200, WB: 1:1000 |
| Reactivity | Mouse |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Affinity purified Anti-NGP antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein of mouse NGP protein. |
| Purity | Anti-NGP is directed against full-length mouse NGP. This product is an affinity purified antibody produced by immunoaffinity chromatography using recombinant protein coupled to agarose beads. A BLAST analysis was used to suggest reactivity with this protein in NGP based on 100% homology match for the immunogen sequence. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
|---|---|
| Form/Appearance | Liquid (sterile filtered) |
| Buffer/Preservatives | Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Concentration | 1.10 mg/ml |
| Dry Ice Shipping | Please note: This product requires shipment on dry ice. A dry ice surcharge will apply. |
| Disclaimer | For research use only |
Alternative Names
−rabbit anti-NGP antibody, Neutrophilic granule protein, Cystatin-like protein, Myeloid bactenecin protein, Myeloid secondary granule protein
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Immunofluorescence of Rabbit Anti-NGP Antibody. Tissue: myc-tagged NGP over expressed THP-1. Fixation: 4% PFA. Primary Antibody: Anti-NGP at 1:200 at RT for 2hrs. Secondary Antibody: rabbit secondary at 1:1000 at RT for 2mins. Staining: NGP is stained red, myc-tag is stained green, and the nucleus is counterstained blue with DAPI. Localization: NGP (red) and myc-tag (green) were colocalized at perinuclear region.
Western Blot of Rabbit anti-Ngp antibody. Lane 1: MW ladder opal pre-stained. Lane 2: 32D lysate. Load: 10 µg per lane. Primary antibody: Ngp antibody at 1:1000 for overnight at 4°C. Secondary antibody: rabbit secondary HRP antibody (p/n orb347654) at 1:70000 for 45 min at RT. Block: BlockOut (p/n orb348644) overnight at 4°C. Predicted/Observed size: 17, 20 kDa for Ngp. Higher banding due to glycosylation. ~125 kDa non-specific.
Western Blot Results of Rabbit Anti-NGP Antibody. Lane 1: Mouse peritoneal neutrophils from NGP deficient mice. Lane 2: Mouse peritoneal neutrophils from wild type. Load: 5 µg total lysate. Primary Antibody: Anti-NGP at 1:1000 at RT for 2hrs. Secondary Antibody: rabbit HRP secondary at 1:20000 at RT for 1hr. Blocking: 5% non-fat dry milk for 30 mins at RT. Predicted/Observed size: 17, 20 kDa for NGP. Glycosylation shows shifting of MW. Results: detects endogenous NGP expressed in mouse neutrophils.
Quick Database Links
UniProt
RefSeq (Protein):NP_032720.2
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_032720.2 |
|---|
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Protocol Information
WB
Western Blot (IB, immunoblot)
IF
Immunofluorescence
Ngp Antibody (orb420373)
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