OCLN/Occludin Antibody

SKU: orb1537178

Description

Images & Validation

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Item 1 of 3

Tested Applications	ELISA, IF, IHC, IHC-P, WB
Dilution range	ELISA, IF, IHC, IHC-P (5 µg/ml), WB (1 - 2 µg/ml)
Reactivity	Human, Mouse, Rat
Application Notes	Further information: OCLN antibody can be used for detection of OCLN by Western blot at 1 ug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 ug/mL. For immunofluorescence start at 20 ug/mL. Antibody validated: Western Blot in human samples; Immunohistochemistry in human and mouse samples and Immunofluorescence in human and mouse samples. All other applications and species not yet tested. Western Blot: Predicted: 57 kDa Observed: 57 kDa Read more...

Key Properties

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Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	A 15 AA synthetic peptide near the C-Terminus of human OCLN. The immunogen is located within the last 50 AA of OCLN.
Target	OCLN / Occludin
Purification	Peptide Column Affinity Chromatography
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	PBS containing 0.02% Sodium Azide
Concentration	1 mg/ml
Disclaimer	For research use only

Alternative Names

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OCLN, Occludin, BLCPMG

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Human Thryoid: Formalin-Fixed, Paraffin-Embedded (FFPE)

Immunofluorescence of OCLN in human liver tissue with OCLN antibody at 20 µg/ml.

Western blot analysis of OCLN in human liver tissue lysate with OCLN antibody at 1 µg/mL.

Quick Database Links

Gene Symbol

OCLN / Occludin

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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IHC-P

Immunohistochemistry Paraffin

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Immunofluorescence

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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