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PCNA Antibody
SKU: orb2643638
Description
Images & Validation
−Item 1 of 3
| Tested Applications | IHC-P, WB |
|---|---|
| Dilution range | Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml |
| Reactivity | Human, Mouse, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | Mouse IgG1, kappa |
| Clone No. | PC5 |
| Immunogen | A rat PCNA/protein A fusion protein was used at the immunogen for this PCNA antibody. |
| Purification | Protein G affinity chromatography |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Disclaimer | For research use only |
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IHC staining of FFPE human tonsil with PCNA antibody (clone PC5). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human colon carcinoma with PCNA antibody (clone PC5). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Western blot testing of human samples with PCNA antibody (clone PC5). Expected molecular weight: 29-36 kDa.
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UniProt
UniProt Details
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
PCNA Antibody (orb2643638)
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