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Rabbit anti-DOT1L Antibody
SKU: orb1529333
Description
Images & Validation
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| Tested Applications | ChIP, IHC, IP, WB |
|---|---|
| Dilution range | WB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate; IHC - 1:200 - 1:1,000. Epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections. |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Immunogen | Between 1000 and 1050 |
| Target | DOT1L |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | 2 - 8°C |
|---|---|
| Form/Appearance | Whole IgG |
| Buffer/Preservatives | Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide |
| Concentration | 200 µg/ml |
| Disclaimer | For research use only |
Alternative Names
−H3-K79-HMTase; histone H3-K79 methyltransferase; lysine N-methyltransferase 4; KMT4; histone methyltransferase DOT1L; DOT1; DOT1-like protein; histone-lysine N-methyltransferase, H3 lysine-79 specific; DOT1-like histone methyltransferase; DOT1 like histone H3K79 methyltransferase; DOT1-like, histone H3 methyltransferase
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Detection of human DOT1L by immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-DOT1L used at a dilution of 1:200 (1µg/ml).
Detection of human DOT1L by WB and IP.Samples: HeLa cells. Antibodies: Affinity purified rabbit anti-DOT1L antibody orb1529333 used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate (B).
Localization of DOT1L Binding Sites by ChIP-sequencing. Chromatin from KOPN-8 cell line was immunoprecipitated with anti-DOT1L antibody and analyzed by DNA sequencing.
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_115871.1 |
|---|
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IP
Immunoprecipitation
ChIP
Chromatin Immunoprecipitation
Rabbit anti-DOT1L Antibody (orb1529333)
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