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Rabbit anti-EHMT1 Antibody
SKU: orb1528040
Description
Images & Validation
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| Tested Applications | IHC, IP, WB |
|---|---|
| Dilution range | WB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate; IHC - 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Immunogen | Between 50 and 100 |
| Target | EHMT1 |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | 2 - 8°C |
|---|---|
| Form/Appearance | Whole IgG |
| Buffer/Preservatives | Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide |
| Concentration | 200 µg/ml |
| Disclaimer | For research use only |
Alternative Names
−G9a-like protein 1; KLEFS1; histone-lysine N-methyltransferase, H3 lysine-9 specific 5; histone-lysine N-methyltransferase EHMT1; histone H3-K9 methyltransferase 5; H3-K9-HMTase 5; lysine N-methyltransferase 1D; KMT1D; FP13812; Eu-HMTase1; EUHMTASE1; euchromatic histone-lysine N-methyltransferase 1; EHMT1-IT1; EHMT1 intronic transcript 1; GLP; GLP1
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Detection of human EHMT1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, A-549, K-562, and MCF-7 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-EHMT1 antibody (orb1528040) used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of human EHMT1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 10% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-EHMT1 antibody (orb1528040) used for IP at 6 µg per reaction. EHMT1 was also immunoprecipitated by a previous lot of this antibody (orb1528040) and a second antibody against a different epitope of EHMT1.
Detection of human EHMT1 by immunohistochemistry.Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-EHMT1 (orb1528040) used at a dilution of 1: 200 (1µg/ml). Detection: DAB
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_079033.3 |
|---|
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IP
Immunoprecipitation
Rabbit anti-EHMT1 Antibody (orb1528040)
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