Rabbit anti-Histone H3 Antibody

SKU: orb1529568

Description

Histone H3 Antibody

Images & Validation

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Tested Applications	IHC, IP, WB
Dilution range	WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:200 - 1:1,000. Epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections.; IHC-IF - 1:100 - 1:500
Reactivity	Human, Mouse
Application Notes	Format: Whole IgG

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Immunogen	Between 86 and C-terminus
Target	Histone H3
Conjugation	Unconjugated

Storage & Handling

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Storage	2 - 8°C
Form/Appearance	Whole IgG
Buffer/Preservatives	Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
Concentration	200 µg/ml
Disclaimer	For research use only

Alternative Names

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Histone H3/c; Histone H3/k; Histone H3/l; Histone H3/j; Histone H3/i; Histone H3/h; Histone H3/f; Histone H3/d; H3/A; histone H3/a; histone H3.1; histone cluster 1, H3a; histone 1, H3a; H3FA; H3 histone family, member A; Histone H3/b

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Detection of human Histone H3 by western blot.Samples: Histones isolated from HeLa cells. Antibody: Affinity purified rabbit anti-Histone H3 antibody orb1529568 at 0.1 µg/ml.

Detection of mouse Histone-H3 by western blot.Samples: Whole cell lysate from TCMK-1 and NIH 3T3 cells prepared using RIPA lysis buffer. Antibody: Affinity purified rabbit anti-Histone-H3 antibody orb1529568 at 0.1 µg/ml.

Detection of human Histone H3 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-Histone H3 used at a dilution of 1:1, 000 (0.2µg/ml).

Detection of human Histone H3 by western blot of immunoprecipitates.Samples: Histones isolated from HeLa cells. Antibodies: Affinity purified rabbit anti-Histone H3 antibody.

IHC: Sample: human prostate carcinoma. Antibody: Affinity purified rabbit anti-Histone H3 used at a dilution of 1:200. Detection: Red-fluorescent goat anti-rabbit IgG-heavy and light chain, cross-adsorbed Antibody DyLight® 594 Conjugated.

Quick Database Links

Gene Symbol

Histone H3

UniProt

P68431

RefSeq (Protein):NP_003520.1

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_003520.1

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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Immunoprecipitation

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