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Rabbit anti-HSP60 Antibody
SKU: orb1525894
Description
Images & Validation
−Item 1 of 2
| Tested Applications | IHC, IP, WB |
|---|---|
| Dilution range | WB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate; IHC - 1:200 - 1:1,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. |
| Reactivity | Human, Mouse |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Immunogen | Between 75 and 125 |
| Target | HSP60 |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | 2 - 8°C |
|---|---|
| Form/Appearance | Whole IgG |
| Buffer/Preservatives | Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide |
| Concentration | 200 µg/ml |
| Disclaimer | For research use only |
Alternative Names
−HLD4; 60 kDa heat shock protein, mitochondrial; P60 lymphocyte protein; mitochondrial matrix protein P1; HuCHA60; HSP65; HSP-60; HSP60; short heat shock protein 60 Hsp60s1; heat shock protein 65; Heat shock protein 60; heat shock 60kDa protein 1 (chaperonin); GROEL; epididymis secretory sperm binding protein; chaperonin 60; SPG13; 60 kDa chaperonin; CPN60
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Detection of human and mouse HSP60 by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) , HEK293T (T; 50 µg) , and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-HSP60 antibody orb1525894 used for WB at 0.04 µg/ml (A) and 0.4 µg/ml (B) and used for IP at 3 µg/mg lysate. HSP60 was also immunoprecipitated by rabbit anti-HSP60 antibodies, which recognize downstream epitopes. Detection: Chemiluminescence with exposure times of 1 second (A and B).
Detection of human and mouse HSP60 by immunohistochemistry.Sample: FFPE section of human ovarian carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti-HSP60 (Cat. No. orb1525894) used at a dilution of 1: 200 (1µg/ml). Detection: Vector Laboratories ImmPACT NovaRED Peroxidase Substrate
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_002147.2 |
|---|
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IP
Immunoprecipitation
Rabbit anti-HSP60 Antibody (orb1525894)
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