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Rabbit Lactate Dehydrogenase B/LDHB Antibody
SKU: orb1522363
Description
Research Area
Immunology & Inflammation, Metabolism Research
Images & Validation
−Item 1 of 2
| Tested Applications | IP, WB |
|---|---|
| Dilution range | WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate |
| Reactivity | Human, Mouse |
| Predicted Reactivity | Rabbit, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Between 284 and C-terminus |
| Target | Lactate Dehydrogenase B/LDHB |
| Purification | Antigen Affinity Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | 2 - 8°C |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
| Concentration | 1000 µg/ml |
| Disclaimer | For research use only |
Alternative Names
−epididymis secretory protein Li 281; HEL-S-281; lactate dehydrogenase H chain; LDH heart subunit; LDH-B; LDHBD; LDH-H; L-lactate dehydrogenase B chain; renal carcinoma antigen NY-REN-46; testicular secretory protein Li 25; TRG-5
Quality Guarantee
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Detection of human and mouse Lactate Dehydrogenase B by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Lactate Dehydrogenase B antibody orb1522363 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Detection of human Lactate Dehydrogenase B by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Lactate Dehydrogenase B antibody orb1522363 used for IP at 6 µg per reaction.
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_002291.1 |
|---|
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Protocol Information
WB
Western Blot (IB, immunoblot)
IP
Immunoprecipitation
Rabbit Lactate Dehydrogenase B/LDHB Antibody (orb1522363)
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