Rabbit anti-Lamin-A/C Antibody

SKU: orb1524852

Description

Lamin-A/C Antibody

Images & Validation

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Item 1 of 4

Tested Applications	ICC, IHC, IP, WB
Dilution range	WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:200 - 1:1,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.; ICC-IF - 1:100 - 1:500. Formaldehyde fixation is recommended. Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells.
Reactivity	Human, Mouse
Application Notes	Format: Whole IgG

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Immunogen	Between 1 and 50
Target	Lamin-A/C
Conjugation	Unconjugated

Storage & Handling

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Storage	2 - 8°C
Form/Appearance	Whole IgG
Buffer/Preservatives	Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
Concentration	200 µg/ml
Disclaimer	For research use only

Alternative Names

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prelamin-A/C; LDP1; LGMD1B; LMN1; LMNC; LMNL1; lamin; mandibuloacral dysplasia type A; lamin A/C-like 1; PRO1; renal carcinoma antigen NY-REN-32; MADA; CDCD1; LFP; 70 kDa lamin; IDC; CDDC; CMD1A; CMT2B1; epididymis secretory sperm binding protein; FPL; FPLD; FPLD2; HGPS; EMD2

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Detection of human Lamin-A and Lamin-C by western blot and immunoprecipitation. Samples: Whole cell lysate (15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Lamin-A/C antibody orb1524852 used for WB at 0.04 µg/ml (A) and 0.4 µg/ml (B) and used for IP at 6 µg/mg lysate. Lamin-A was also immunoprecipitated by rabbit anti-Lamin-A antibody, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 1 second (A and B).

Detection of mouse Lamin-A /C by immunohistochemistry.Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti-Lamin-A/C (Cat. No. orb1524852) used at a dilution of 1: 200 (1µg/ml). Detection: DAB

Detection of human Lamin-A /C by immunohistochemistry. Samples: FFPE sections of human colon carcinoma. Antibody: Affinity purified rabbit anti-Lamin-A/C (Cat. No. orb1524852) used at a dilution of 1: 200 (1µg/ml) (left) and 1: 1, 000 (0.2µg/ml) (right). Detection: DAB

Detection of human Lamin-A /C by immunocytochemistry. Samples: Formaldehyde-fixed asynchronous HeLa cells. Antibody: Affinity purified rabbit anti-Lamin-A/C (Cat. No. orb1524852) used at a dilution of 1: 200 (1µg/ml). Detection: Red-fluorescent goat anti-rabbit IgG-heavy and light chain cross-adsorbed Antibody DyLight® 594 Conjugated used at a dilution of 1: 100.

Quick Database Links

Gene Symbol

Lamin-A/C

UniProt

P02545

RefSeq (Protein):NP_733821.1

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_733821.1

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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ICC

Immunocytochemistry

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Immunoprecipitation

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